Article

Article name SEED MICROMORPHOLOGY SUPPORTS SPECIES DELIMITATION OF ORCHIS CANARIENSIS (ORCHIDACEAE), AN ENDEMIC ORCHID FROM THE CANARY ISLANDS
Authors

Jacopo Calevo, PhD, Postdoc Researcher at the University of Naples Federico II, Department of Biology (80126, Italy, Naples, Via Cinthia, Campus Monte Sant'Angelo building 7); iD ORCID: https://orcid.org/0000-0002-1717-2365; e-mail: jacopo.calevo@unina.it
Miriam Bazzicalupo, PhD, Research Assistant at the University of Genoa, Department of Earth, Environment and Life Sciences (16132, Italy, Genoa, Corso Europa 26); iD ORCID: https://orcid.org/0000-0002-9785-1981; e-mail: miriam.bazzicalupo@gmail.com
Cristina González-Montelongo, PhD, Postdoc Researcher at the Universidad de La Laguna, Department of Botany, Ecology and Plant Physiology (38200, Spain, Santa Cruz de Tenerife 456); iD ORCID: https://orcid.org/0000-0001-9236-890X; e-mail: cgonzalm@ull.es
Daniel Rodríguez González, Student at the University of Alcalá (UAH), Department of Life Sciences (28871, Spain, Madrid, Alcalá de Henares, Campus Universitario - Edificio Ciencias); e-mail: danitorg94@gmail.com
Laura Cornara, PhD, Associate Professor at the University of Genoa, Department of Earth, Environment and Life Sciences (16132, Italy, Genoa, Corso Europa 26); iD ORCID: https://orcid.org/0000-0001-9154-7789; e-mail: laura.cornara@unige.it

Reference to article

Calevo J., Bazzicalupo M., González-Montelongo C., Rodríguez González D., Cornara L. 2022. Seed micromorphology supports species delimitation of Orchis canariensis (Orchidaceae), an endemic orchid from the Canary Islands. Nature Conservation Research 7(3): 95–101. https://dx.doi.org/10.24189/ncr.2022.031

Section Short Communications
DOI https://dx.doi.org/10.24189/ncr.2022.031
Abstract

Geographic isolation is one of the primary drivers of speciation, but islands remain a reservoir of overlooked and understudied endemic species. In this study, we collected seed micromorphological data from Orchis canariensis (Orchidaceae), a declining species occurring only on the Canary Islands (Spain), whose taxonomy was debated in the past decades. The aim of the study was to detect seed micromorphological traits in support of species delimitation by means of light and scanning electron microscopy. Seeds from a population on Tenerife resulted to be clavate with a cell number in the longitudinal axis varying from five to seven. Seeds showed straight to sinuous anticlinal walls and no ornamentations in the periclinal walls. The average seed length was 313.66 ± 44.78 µm and the average width 184.31 ± 30.26 µm, with a ratio of 1.72 ± 0.25, while the embryo length and width were 157.18 ± 35.21 µm and 125.43 ± 25.92 µm respectively. However, despite the affinities with the sister species, Orchis patens, quantitative and qualitative seed traits supported species delimitation recently proposed by means of molecular biology. Taken together, our results confirmed the importance of seed micromorphology in support of taxonomical studies.

Keywords

endemic species, island flora, orchid conservation, Orchis patens, Red List, scanning electron microscopy, taxonomy, Tenerife

Artice information

Received: 25.03.2022. Revised: 17.07.2022. Accepted: 19.07.2022.

The full text of the article
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