Irfan I. Sofi, University of Kashmir (Srinagar-19006, Jammu and Kashmir, India); e-mail:
Shivali Verma, University of Jammu (Jammu Tawi-18006, Jammu and Kashmir, India); e-mail:
Aijaz H. Ganie, University of Kashmir (Srinagar-19006, Jammu and Kashmir, India); e-mail:
Namrata Sharma, University of Jammu (Jammu Tawi-18006, Jammu and Kashmir, India); e-mail:
Manzoor A. Shah, University of Kashmir (Srinagar-19006, Jammu and Kashmir, India); iD ORCID:; e-mail:

Reference to article

Sofi I.I., Verma S., Ganie A.H., Sharma N., Shah M.A. 2022. Threat status of three important medicinal Himalayan plant species and conservation implications. Nature Conservation Research 7(1): 27–41.

Electronic Supplement. Collection sites of target plants with co-ordinates and guidelines for assessment of overall threat impact (Link).

Section Research articles

A lack of precise information about the threat status of species hampers their effective conservation. The Target 2 of the Convention on Biological Diversity calls for evaluation of threat status at global, national and regional levels to identify plant species of urgent conservation concern. Here we have empirically assessed the threat status of three valuable medicinal plant species (Trillium govanianum, Rheum tibeticum, and Arnebia euchroma) through extensive field studies and herbarium consultations in Kashmir Himalaya and cold desert region of Trans-Himalayan Ladakh. In accordance with the IUCN Red List categories and criteria, each of the three target species turned out to be Near Threatened (NT). According to the NatureServe Conservation Status Assessment, each of these species faces the overall threat impact of «High» to «Very high». We found that the anthropogenic threats emanating from unplanned economic development, road construction and other infrastructure related projects contribute to a fast decline in natural populations of these three species. Keeping in view the value of these species, on the one hand, and growing threats to their survival in the wild, on the other one, we call for urgent conservation strategies in the vulnerable Himalayan habitats for regional socio-economic development.


Arnebia euchroma, Kashmir Himalaya, Near Threatened, overall threat impact, Rheum tibeticum, threat assessment, Trans-Himalaya, Trillium govanianum

Artice information

Received: 08.10.2021. Revised: 02.01.2022. Accepted: 04.01.2022.

The full text of the article

Ali H., Qaiser M. 2010. Contribution to the Red List of Pakistan: a case study of Gaillonia chitralensis (Rubiaceae). Pakistan Journal of Botany 42: 2967–2971.
Badola H.K., Aitken S. 2003. The Himalayas of India: A treasury of medicinal plants under siege. Biodiversity 4(3): 3–13. DOI: 10.1080/14888386.2003.9712694
Baillie J.E.M., Hilton-Taylor C., Stuart S.N. (Eds.). 2004. IUCN red list of threatened species. A Global Species Assessment. Gland, Switzerland and Cambridge, UK: IUCN. 191 p.
Bland L.M., Keith D.A., Miller R.M., Murray N.J., Rodríguez J.P. (Eds.). 2017. Guidelines for the application of IUCN Red List of Ecosystems Categories and Criteria, Version 1.1. Gland, Switzerland: IUCN. 99 p.
Bowler D.E., Bjorkman A.D., Dornelas M., Myers‐Smith I.H., Navarro L.M., Niamir A., Supp S.R., Waldock C., Winter M., Vellend M., Blowes S.A., Böhning-Gaese K., Bruelheide H., Elahi R., Antão L.H., Hines J., Isbell F., Jones H.P., Magurran A.E., Sarmento Cabral J., Bates A.E. 2020. Mapping human pressures on biodiversity across the planet uncovers anthropogenic threat complexes. People and Nature 2(2): 380–394. DOI: 10.1002/pan3.10071
Butchart S.H., Walpole M., Collen B., Van Strien A., Scharlemann J.P., Almond R.E., Carpenter K.E., Carr G.M., Chanson J., Chenery A.M., Csirke J., Davidson N.C., Dentener F., Foster M., Galli A., Galloway J.N., Genovesi P., Gregory R.D., Hockings M., Kapos V., Lamarque J.-F., Leverington F., Loh J., McGeoch M.A., McRae L., Minasyan A., Morcillo M.H., Oldfield T.E.E., Pauly D., Quader S. et al. 2010. Global biodiversity: indicators of recent declines. Science 328(2010): 1164–1168. DOI: 10.1126/science.1187512
Butt T.A., Ganie A.H., Khuroo A.A., Ahmad R., Rasool N., Basharat S. 2020. New distributional record of Primula atrodentata W. W. Sm. from Ladakh region and assessment of its threat status across the Himalaya. Acta Ecologica Sinica 41(3): 183–188. DOI: 10.1016/j.chnaes.2020.08.002
Chauhan H.K., Bisht A.K., Bhatt I.D., Bhatt A., Gallacher D., Santo A. 2018. Population change of Trillium govanianum (Melanthiaceae) amid altered indigenous harvesting practices in the Indian Himalayas. Journal of Ethnopharmacology 213: 302–310. DOI: 10.1016/j.jep.2017.11.003
Chen S.L., Yu H., Luo H.M., Wu Q., Li C.F., Steinmetz A. 2016. Conservation and sustainable use of medicinal plants: problems, progress, and prospects. Chinese Medicine 11(1): 37. DOI: 10.1186/s13020-016-0108-7
Chitale V.S., Behera M.D., Roy P.S. 2015. Global biodiversity hotspots in India: significant yet under studied. Current Science 108(2): 149–150.
Christiansen F.B., Fenchel T.M. 2012. Theories of populations in biological communities. Berlin: Springer Science & Business Media. 144 p.
Collen B., Dulvy N.K., Gaston K.J., Gärdenfors U., Keith D.A., Punt A.E., Regan H.M., Böhm M., Hedges S., Seddon M., Butchart S.H., Hilton-Taylor C., Hoffmann M., Bachman S.P., Akçakaya H.R. 2016. Clarifying misconceptions of extinction risk assessment with the IUCN Red List. Biology Letters 12(4): 20150843. DOI: 10.1098/rsbl.2015.0843
Darbyshire I., Timberlake J., Osborne J., Rokni S., Matimele H., Langa, C., Datizua C., de Sousa C., Alves T., Massingue A., Hadj-Hammou, J. 2019. The endemic plants of Mozambique: diversity and conservation status. PhytoKeys 136: 45–96. DOI:10.3897/phytokeys.136.39020
Dauby G., Stévart T., Droissart V., Cosiaux A., Deblauwe V., Simo‐Droissart M., Sosef M.S., Lowry P.P., Schatz G.E., Gereau R.E., Couvreur T. 2017. ConR: An R package to assist large-scale multispecies preliminary conservation assessments using distribution data. Ecology and Evolution 7(24): 11292–11303. DOI: 10.1002/ece3.3704
Dawa S., Gurmet P., Dolma T., Angdus T., Stobgais T., Tharpa T. 2018. Status of Medicinal and Aromatic Plants in the State of Jammu & Kashmir. International Journal of Current Microbiology and Applied Sciences 7(12): 2597–2615. DOI: 10.20546/ijcmas.2018.712.295
Dhyani P., Sharma B., Singh P., Masand M., Seth R., Sharma R.K. 2020. Genome-wide discovery of microsatellite markers and, population genetic diversity inferences revealed high anthropogenic pressure on endemic populations of Trillium govanianum. Industrial Crops and Products 154: 112698. DOI: 10.1016/j.indcrop.2020.112698
Ganie A.H., Tali B.A., Reshi Z.A., Nawchoo I.A. 2017. Stigmatic Movement Promotes Cross Pollination in Rheum webbianum Royle: An Important Endemic Medicinal Plant of Kashmir Himalaya. National Academy Science Letters 40(6): 435–438. DOI: 10.1007/s40009-017-0579-9
Ganie A.H., Tali B.A., Khuroo A.A, Reshi Z.A., Nawchoo I.A. 2019. Impact assessment of anthropogenic threats to high-valued medicinal plants of Kashmir Himalaya, India. Journal for Nature Conservation 50: 125715. DOI: 10.1016/j.jnc.2019.125715
Gaston K.J., Fuller R.A. 2009. The sizes of species' geographic ranges. Journal of Applied Ecology 46(1): 1–9. DOI: 10.1111/j.1365-2664.2008.01596.x
Gauthier P., Debussche M., Thompson J.D. 2010. Regional priority setting for rare species based on a method combining three criteria. Biological Conservation 143(6): 1501–1509. DOI: 10.1016/j.biocon.2010.03.032
Heywood V.H. 2017. Plant conservation in the Anthropocene – challenges and future prospects. Plant Diversity 39(6): 314–330. DOI: 10.1016/j.pld.2017.10.004
Hosseini A., Mirzaee F., Davoodi A., Jouybari H.B., Azadbakht M. 2018. The traditional medicine aspects, biological activity and phytochemistry of Arnebia spp. Medicinski Glasnik 15(1): 1–9. DOI: 10.17392/926-18
Hussain M. 2002. Geography of Jammu and Kashmir. New Delhi, India: Rajesh Publications. 186 p.
IUCN. 2001. IUCN Red List Categories and Criteria: Version 3.1. Gland, Switzerland; Cambridge, UK: IUCN. 32 p.
IUCN. 2012a. Guidelines for Application of IUCN Red List Criteria at Regional and National Levels: Version 4.0. Gland, Switzerland; Cambridge, UK: IUCN. 41 p.
IUCN. 2012b. IUCN Red List Categories and Criteria: Version 3.1. Second edition. Gland, Switzerland; Cambridge, UK: IUCN. 32 p.
IUCN. 2019. Guidelines for Using the IUCN Red List Categories and Criteria. Version 14. Prepared by the Standards and Petitions Committee. Available from:
Johnson C.N., Balmford A., Brook B.W., Buettel J.C., Galetti M., Guangchun L., Wilmshurst J.M. 2017. Biodiversity losses and conservation responses in the Anthropocene. Science 356(6335): 270–275. DOI: 10.1126/science.aam9317
Kumar G., Gupta S., Murugan M., Bala-Singh S. 2009. Ethnobotanical studies of Nubra Valley – A cold arid zone of Himalaya. Ethnobotanical Leaflets 2009(6): 9.
Lal M., Samant S.S., Kumar R., Sharma L., Paul S., Dutt S., Negi D., Devi K. 2020. Population ecology and niche modelling of endangered Arnebia euchroma in Himachal Pradesh, India – An approach for conservation. Medicinal Plants – International Journal of Phytomedicines and Related Industries 12(1): 90–104. DOI: 10.5958/0975-6892.2020.00013.1
Le Breton T.D., Zimmer H.C., Gallagher R.V., Cox M., Allen S., Auld T.D. 2019. Using IUCN criteria to perform rapid assessments of at-risk taxa. Biodiversity and Conservation 28(4): 863–883. DOI: 10.1007/s10531-019-01697-9
Le Roux J.J., Hui C., Castillo M.L., Iriondo J.M., Keet J.H., Khapugin A.A., Médail F., Rejmánek M., Theron G., Yannelli F.A., Hirsch H. 2019. Recent Anthropogenic Plant Extinctions Differ in Biodiversity Hotspots and Coldspots. Current Biology 29(17): 2912–2918. DOI: 10.1016/j.cub.2019.07.063
Manjkhola S., Dhar U., Joshi M. 2005. Organogenesis, embryogenesis, and synthetic seed production in Arnebia euchroma – A critically endangered medicinal plant of the Himalaya. In Vitro Cellular and Developmental Biology – Plant 41(3): 244–248. DOI: 10.1079/IVP2004612
Maron M., Hobbs R.J., Moilanen A., Matthews J.W., Christie K., Gardner T.A., Keith D.A., Lindenmayer D.B., McAlpine C.A. 2012. Faustian bargains? Restoration realities in the context of biodiversity offset policies. Biological Conservation 155: 141–148. DOI: 10.1016/j.biocon.2012.06.003
Master L.L., Faber-Langendoen D., Bittman R., Hammerson G.A., Heidel B., Ramsay L., Snow K., Teucher A., Tomaino A. 2012. NatureServe conservation status assessments: Factors for evaluating species and ecosystems at risk. Arlington, VA: NatureServe. Available from:
Mehta P., Sekar K.C., Bhatt D., Tewari A., Bisht K., Upadhyay S., Negi V.S., Soragi B. 2020. Conservation and prioritization of threatened plants in Indian Himalayan Region. Biodiversity and Conservation 29(6): 1723–1745. DOI: 10.1007/s10531-020-01959-x
Meng H., Gao X., Song Y., Cao G., Li J. 2021. Biodiversity arks in the Anthropocene. Regional Sustainability 2(2): 109–115. DOI: 10.1016/j.regsus.2021.03.001
Myers N., Mittermeier R.A., Mittermeier C.G., Da Fonseca, Kent J. 2000. Biodiversity hotspots for conservation priorities. Nature 403(6772): 853–858. DOI: 10.1038/35002501
Olsen C.S., Larsen H.O. 2003. Alpine Medicinal Plant Trade and Himalayan Mountain Livelihood Strategies. Geographical Journal 169(3): 243–254. DOI: 10.1111/1475-4959.00088
Orsenigo S., Montagnani C., Fenu G., Gargano D., Peruzzi L., Abeli T., Alessandrini A., Bacchetta G., Bartolucci F., Bovio M., Brullo C., Brullo S., Carta A., Castello M., Cogoni D., Conti F., Domina G., Foggi B., Gennai M., Gigante D., Iberite M., Lasen C., Magrini S., Perrino E.V., Prosser F., Santangelo A., Selvaggi A., Stinca A., Vagge I., Villani M. et al. 2018. Red Listing plants under full national responsibility: Extinction risk and threats in the vascular flora endemic to Italy. Biological Conservation 224: 213–222. DOI: 10.1016/j.biocon.2018.05.030
Rao R.R. 2019. Medicinal Plants of India: Diversity, Conservation and Bioprospection-concerns and strategies for 21st century. TTPP. P.1.
Rathore S., Walia S., Devi R., Kumar R. 2020. Review on Trillium govanianum Wall. ex D. Don: A threatened medicinal plant from the Himalaya. Journal of Herbal Medicine 24: 100395. DOI: 10.1016/j.hermed.2020.100395
Rawal R.S., Negi V.S., Bhatt I.D. 2021. Changing Outlook on Harnessing Biodiversity Values – A Special Focus on Indian Himalaya. Journal of Graphic Era University 9(1): 55–82. DOI: 10.13052/jgeu0975-1416.914
Reid A.J., Carlson A.K., Creed I.F., Eliason E.J., Gell P.A., Johnson P.T., Kidd K.A., MacCormack T.J., Olden J.D., Ormerod S.J., Smol J.P., Taylor W.W., Tockner K., Vermaire J.C., Dudgeon D., Cooke S.J. 2019. Emerging threats and persistent conservation challenges for freshwater biodiversity. Biological Reviews 94(3): 849–873. DOI:10.1111/brv.12480
Rivers M.C., Bachman S.P., Meagher T.R., Lughadha E.N., Brummitt N.A. 2010. Subpopulations, locations and fragmentation: applying IUCN red list criteria to herbarium specimen data. Biodiversity and Conservation 19(7): 2071–2085. DOI: 10.1007/s10531-010-9826-9
Robles H., Ciudad C. 2012. Influence of habitat quality, population size, patch size, and connectivity on patch-occupancy dynamics of the middle-spotted woodpecker. Conservation Biology 26(2): 284–293. DOI: 10.1111/j.1523-1739.2011.01816.x
Romshoo S.A., Rashid I., Altaf S., Dar G.H. 2020. Jammu and Kashmir State: An Overview. In: G.H. Dar, A.A. Khuroo (Eds.): Biodiversity of the Himalaya: Jammu and Kashmir State. Topics in Biodiversity and Conservation. Vol. 18. Singapore: Springer. P. 129–166. DOI: 10.1007/978-981-32-9174-4_6
Roy P.S., Roy A., Joshi P.K., Kale M.P., Srivastava V.K., Srivastava S.K., Dwevidi R.S., Joshi C., Behera M.D., Meiyappan P., Sharma Y., Jain A.K., Singh J.S., Palchowdhuri Y., Ramachandran R.M., Pinjarla B., Chakravarthi V., Babu N., Gowsalya M.S., Thiruvengadam P., Kotteeswaran M., Priya V., Yelishetty K.M.V.N., Maithani S., Talukdar G., Mondal I., Rajan K.S., Narendra P.S., Biswal S., Chakraborty A. et al. 2015. Development of decadal (1985–1995–2005) land use and land cover database for India. Remote Sensing 7(3): 2401–2430. DOI: 10.3390/rs70302401
Salafsky N., Salzer D., Stattersfield A.J., Hilton-Taylor C., Neugarten R., Butchart S.H.M., Collen B., Cox N., Master L.L., O'Connor S., Wilkie D. 2008. A standard lexicon for biodiversity conservation: unified classifications of threats and actions. Conservation Biology 22(4): 897–911. DOI: 10.1111/j.1523-1739.2008.00937.x
Samant S.S., Dhar U., Palni L.M.S. 1998. Medicinal Plants of Indian Himalaya: Diversity, Distribution Potential Values. Nainital, India: Gyanodaya Prakashan. 163 p.
Schippmann U.W.E., Leaman D., Cunningham A.B. 2006. A comparison of cultivation and wild collection of medicinal and aromatic plants under sustainability aspects. Frontis 17: 75–95.
Schmeller D.S., Gruber B., Budrys E., Framsted E., Lengyel S., Henle K. 2008. National Responsibilities in European Species Conservation: a Methodological Review. Conservation Biology 22(3): 593–601. DOI: 10.1111/j.1523-1739.2008.00961.x
Sequeira A.M., Bouchet P.J., Yates K.L., Mengersen K., Caley M.J. 2018. Transferring biodiversity models for conservation: opportunities and challenges. Methods in Ecology and Evolution 9(5): 1250–1264. DOI: 10.1111/2041-210X.12998
Sharma A., Sharma A. 2019. Impact of anthropogenic activities on Himalayan ecosystem services and their management/sustainability: A review. The Pharma Innovation International Journal 8(1): 361–365.
Shepherd E., Milner‐Gulland E.J., Knight A.T., Ling M.A., Darrah S., van Soesbergen A., Burgess N.D. 2016. Status and trends in global ecosystem services and natural capital: assessing progress toward Aichi Biodiversity Target 14. Conservation Letters 9(6): 429–437. DOI: 10.1111/conl.12320
Singh H., Chauhan R., Raina R. 2017. Population structure, ecological features and associated species of Arnebia euchroma. Journal of Pharmacognosy and Phytochemistry 6(4): 2005–2007.
Singh K.N., Lal B., Chand G., Todaria N.P. 2012. Ecological features and conservation of Arnebia euchroma. A critically endangered medicinal plant in western Himalaya. International Journal of Conservation Science 3(3): 189–198.
Singh K.K., Singh S.P., Kumari B., Paliwal A.K., Chauhan J.S., Rawat J.M.S. 2019. Income Generation of Farmers via Medicinal Plants Cultivation: A Review. International Journal of Pure and Applied Biosciences 7(4): 174–177. DOI: 10.18782/2320-7051.7428
Staude I.R., Navarro L.M., Pereira H.M. 2020. Range size predicts the risk of local extinction from habitat loss. Global Ecology and Biogeography 29(1): 16–25. DOI: 10.1111/geb.13003
Tali B.A., Ganie A.H., Nawchoo I.A., Wani A.A., Reshi Z.A. 2015. Assessment of threat status of selected endemic medicinal plants using IUCN regional guidelines: A case study from Kashmir Himalaya. Journal for Nature Conservation 23: 80–89. DOI: 10.1016/j.jnc.2014.06.004
Tali B.A., Khuroo A.A., Nawchoo I.A., Ganie A.H. 2018. Prioritizing conservation of medicinal flora in the Himalayan biodiversity hotspot: an integrated ecological and socioeconomic approach. Environmental Conservation 46(2): 147–154. DOI: 10.1017/S0376892918000425
Turvey S.T., Crees J.J. 2019. Extinction in the Anthropocene. Current Biology 29(19): R982–R986. DOI: 10.1016/j.cub.2019.07.040
Underwood E.C., Viers J.H., Klausmeyer K.R., Cox R.L., Shaw M.R. 2009. Threats and biodiversity in the Mediterranean biome. Diversity and Distributions 15(2): 188–197. DOI: 10.1111/j.1472-4642.2008.00518.x
Vidyarthi S., Samant S.S., Sharma P. 2013. Dwindling status of Trillium govanianum Wall. ex D. Don – A case study from Kullu district of Himachal Pradesh, India. Journal of Medicinal Plants Research 7(8): 392–397. DOI: 10.5897/JMPR12.622
Zhang Q., Cai D., Wang L., Yang X., Fan S., Zhang K. 2018. Rapid and sensitive determination of shikonin and its derivatives in the roots of Arnebia euchroma (Royle) Johnst using matrix solid-phase dispersion extraction and ultrahigh-performance liquid chromatography with photodiode array detector. Journal of Liquid Chromatography and Related Technologies 41(9): 489–497. DOI: 10.1080/10826076.2018.1467836