Article

Article name CONSERVATION ISSUES AND INFRASPECIFIC POLYMORPHISM OF CYPRIPEDIUM GUTTATUM ON SELECTED LOCATIONS IN RUSSIA
Authors

Alexander I. Shirokov, PhD, Director of the Botanical Garden, Associate Professor of the Department of Botany and Zoology in the Lobachevsky State University of Nizhny Novgorod (603950, Russia, Nizhny Novgorod, Gagarin Avenue, 23); e-mail: aishirokov@mail.ru
Vera V. Syrova, PhD, Associate Professor of the Department of Botany and Zoology, Head of laboratory of Botanical Garden in the Lobachevsky State University of Nizhny Novgorod (603950, Russia, Nizhny Novgorod, Gagarin Avenue, 23); e-mail: vvsyrova@mail.ru
Alexey V. Salokhin, PhD, Research Fellow of Botanical Garden-Institute FEB RAS (690024, Russia, Vladivostok, Makovskii Str. 142); e-mail: al-xv@mail.ru
Ivan N. Markelov, PhD, Leading Scientist-Biologist of Botanical Garden in the Lobachevsky State University of Nizhny Novgorod (603950, Russia, Nizhny Novgorod, Gagarin Avenue, 23); e-mail: ivamark777@gmail.com
Elena V. Andronova, PhD, Senior Research Fellow in Komarov Botanical Institute of RAS (197376, Russia, St. Petersburg, Professor Popov Street, 2); e-mail: elena.andronova@mail.ru
Elena V. Ganyushkina, Senior Teacher of the Department of English for Humanities in Lobachevsky State University (603950, Russia, Nizhny Novgorod, Gagarin Avenue, 23); e-mail: elenaganyushkina@gmail.com

Reference to article

Shirokov A.I., Syrova V.V., Salokhin A.V., Markelov I.N., Andronova E.V., Ganyushkina E.V. 2020. Conservation issues and infraspecific polymorphism of Cypripedium guttatum on selected locations in Russia. Nature Conservation Research 5(Suppl.1): 145–154. https://dx.doi.org/10.24189/ncr.2020.054

Section Research articles
DOI https://dx.doi.org/10.24189/ncr.2020.054
Abstract

The problem of protection and conservation of rare and endangered plant species in the wild is currently the topic of discussion and research worldwide. The Orchidaceae plants are of special concern. Orchids are so well adapted to their environments. Moreover, they have special requirements that makes it difficult to transplant them from the wild and they are rarely surviving after. They are therefore a key group in conservation. This research aimed to study the polymorphism of Cypripedium guttatum flowers in their natural populations on selected locations in Russia. On the basis of studying C. guttatum populations in Primorsky Krai, Sverdlovsk region, Komi Republic, and Krasnoyarsky Krai, we identified and described five forms of C. guttatum in terms of their flower colouration. A comparison study of the infraspecific structure revealed that the form variation in the studied C. guttatum populations was the highest in Primorsky Krai and the lowest in Sverdlovsk region. A discriminant analysis indicated the following statistically significant morphometric parameters to recognise different forms of C. guttatum: shoot height, leaf width, flower width, height and width of dorsal sepal and staminode width. A population variation analysis was conducted with respect to the following three indicators: the average number of morphs per population, the rare morph ratio in the population, and the similarity index of the studied populations. On the basis of monitoring C. guttatum populations and studying their infraspecific structure, as well as the biology and ecology of this threatened orchid species, we developed a specific conservation project for rare and endangered orchid species complying with the unique biology of these plants.

Keywords

flower colouration, discriminant analysis, morphometric parameters, monitoring, Orchidaceae, rare species, specific project

Artice information

Received: 14.04.2020. Revised: 22.08.2020. Accepted: 01.09.2020.

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References

Afanasieva E.A., Galkina M.A., Kazantseva E.S. 2013. Cenopopulation of Cypripedium guttatum Sw. in the Central Yakutia. Vestnik of North-Eastern Federal University 10(4): 11–18. [In Russian]
Averyanov L.V. 1999. Genus Cypripedium (Orchidaceae) in the Russia. Turczaninowia 2(2): 5–40. [In Russian]
Averyanov L.V. 2000. Orchids (Orchidaceae) of the Middle Russia. Turczaninowia 3(1): 30–53. [In Russian]
Bänziger H., Sun H.Q., Luo Y.B. 2005. Pollination of a slippery lady slipper orchid in South-West China: Cypripedium guttatum (Orchidaceae). Botanical Journal of the Linnean Society 148(3): 251–264. DOI: 10.1111/j.1095-8339.2005.00400.x
Biryukova O.V., Shestakova A.A., Vorotnikov V.P., Bakka S.V. 2018. Rare and protected species of orchids in the Protected Areas of the Nizhny Novgorod region. In: Protection and cultivation of orchids. Nizhny Novgorod: Nizhny Novgorod University. P. 47–48. [In Russian]
Cribb P. 1997. The genus Cypripedium. Portland: Timber Press. 301 p.
Cribb P., Sandison M.S. 1998. A preliminary assessment of the conservation status of Cypripedium species in the wild. Botanical Journal of the Linnean Society 126(1–2): 183–190. DOI: 10.1111/j.1095-8339.1998.tb02525.x
Cribb P.J., Kell S.P., Dixon K.W., Barrett R.L. 2003. Orchid conservation: a global perspective. In: K.W. Dixon, S.P. Kell, R.L. Barrett, P.J. Cribb (Eds.): Orchid Con-servation. Kota Kinabalu: Natural History Publications. P. 1–24.
Dafni A., Tzohari H., Ben-Shlomo R., Vereecken N.J., Ne'eman G. 2020. Flower Colour Polymorphism, Pollination Modes, Breeding System and Gene Flow in Anemone coronaria. Plants 9(3): 397. DOI: 10.3390/plants9030397
Dickson C.R., Petit S. 2006. Effect of individual height and labellum colour on the pollination of Caladenia (syn. Arachnorchis) behrii (Orchidaceae) in the northern Adelaide region, South Australia. Plant Systematics and Evolution 262(1): 65–74. DOI: 10.1007/s00606-006-0472-3
Fay M.F. 2018. Orchid conservation: how can we meet the challenges in the twenty-first century? Botanical Studies 59: 16. DOI: 10.1186/s40529-018-0232-z
Feldmann P., Prat D. 2011. Conservation recommendations from a large survey of French orchids. European Journal of Environmental Sciences 1(2): 18–27. DOI: 10.14712/23361964.2015.43
Flora of North America North of Mexico. Vol. 26: Magnoliophyta: Liliidae: Liliales and Orchidales. New York: Oxford University Press, 2002. 752 p.
Galen C. 1999. Why Do Flowers Vary? The functional ecology of variation in flower size and form within natural plant populations. Bioscience 49(8): 631–640. DOI: 10.2307/1313439
Gigord L., Macnair M., Smithson A. 2001. Negative frequency-dependent selection maintains a dramatic flower colour polymorphism in the rewardless orchid Dactylorhiza sambucina (L.) Soò. Proceedings of the National Academy of Sciences of the United States of America 98(11): 6253–6255. DOI: 10.1073/pnas.111162598
Hardion L., Verlaque R., Vorontsova M.S., Combroux I., Chen C.W., Takamizo T., Vila B. 2017. Does infraspecific taxonomy match species evolutionary history? A phylogeographic study of Arundo formosana (Poaceae). Botanical Journal of the Linnean Society 183(2): 236–249. DOI: 10.1093/botlinnean/bow006ff
Juillet N., Delle-Vedove R., Dormont L., Schatz B., Pailler T. 2010. Differentiation in a tropical deceptive orchid: Colour polymorphism and beyond. Plant Systematics and Evolution 289(3): 213–221. DOI: 10.1007/s00606-010-0344-8
Kawecki T.J. 2008. Adaptation to Marginal Habitats. Annual Review of Ecology, Evolution, and Systematics 39: 321–342. DOI: 10.1146/annurev.ecolsys.38.091206.095622
Kay Q.O.N. 1978. The Pollination of Flowers by Insects. Linnean Society Symposium Series 6: 175–190.
Kolanowska M., Jakubska-Busse A. 2020. Is the lady's-slipper orchid (Cypripedium calceolus) likely to shortly become extinct in Europe? – Insights based on ecological niche modelling. PLoS ONE 15(1): e0228420. DOI: 10.1371/journal.pone.0228420
Kopowitz H. 2005. Orchid conservation and CITES. In: Raynal-Roque, A. Roguenant, D. Prat (Eds.): Proceedings of the 18th World Orchid Conference (11–20 March 2005, Dijon-France). Turriers: Naturalia. P. 301–305.
Li J.H., Liu Z.J., Salazar G.A., Bernhardt P., Perner H., Tomohisa Y., Jin X.H., Chung S.W., Luo Y.B. 2011. Molecular phylogeny of Cypripedium (Orchidaceae: Cypripedioideae) inferred from multiple nuclear and chloroplast regions. Molecular Phylogenetics and Evolution 61(2): 308–320. DOI: 10.1016/j.ympev.2011.06.006
Marakaev O.A. 2016. Orchids (Orchidaceae Juss.) at especially protected natural territories of Yaroslavl oblast. Proceedings of Samara Scientific Centre RAS 18(2): 136–140. [In Russian]
Red Data Book of the Russian Federation (plants and fungi). Moscow: KMK Scientific Press Ltd., 2008. 855 p. [In Russian]
Robertson J.L., Wyatt R. 1990. Evidence for pollination ecotypes in the yellow-fringed orchid, Platanthera ciliaris. Evolution 44(1): 121–133. DOI: 10.1111/j.1558-5646.1990.tb04283.x
Salokhin A.V. 2009. Orchidaceae of the Far East (taxonomy, chemical composition and use possibilities). PhD Thesis. Vladivostok. 24 p. [In Russian]
Salokhin A.V. 2018. Features of the distribution of orchids in the Far East. In: Protection and cultivation of orchids. Nizhny Novgorod: Nizhny Novgorod University. P. 74–75. [In Russian]
Shirokov A.I., Salokhin A.V., Syrova V.V., Kryukov L.A. 2014. Results of creating a collection of the Cypripedium genus at the UNN Botanical Garden Research Institute. Vestnik of Lobachevsky University of Nizhni Novgorod 3(3): 150–153. [In Russian]
Shirokov A.I., Syrova V.V., Kryukov L.A., Shestakova A.A. 2017. Reintroduction of Dactylorhiza incarnata (L.) Soó into the natural habitats of the European Russia. Applied Ecology and Environmental Research 15(1): 445–455. DOI: 10.15666/aeer/1501_445455
Singchi C., Zhongjian L., Lijun C., Liqiang L. 2013. The Genus Cypripedium in China. Beijing: Science Press. 317 p.
Stuessy T.F. 2009. Plant Taxonomy: The Systematic Evaluation of Comparative Data. New York: Columbia University Press. 568 p.
Subramaniam B., Rausher M.D. 2000. Balancing selection on a floral polymorphism. Evolution 54(2): 691–695. DOI: 10.1111/j.0014-3820.2000.tb00070.x
Swarts N.D., Dixon K.W. 2009a. Perspectives on orchid conservation in botanic gardens. Trends in Plant Science 14(11): 590–598. DOI: 10.1016/j.tplants.2009.07.008
Swarts N.D., Dixon K.W. 2009b. Terrestrial orchid conservation in the age of extinction. Annals of Botany 104(3): 543–556. DOI: 10.1093/aob/mcp025
Swarts N.D., Dixon K.W. 2017. Conservation methods for terrestrial orchids. Plantation: J. Ross Publishing. 240 p.
Thompson J.D., Chalchat J.C., Michet A., Linhart Y.B., Ehlers B. 2003. Qualitative and quantitative variation in monoterpene co-occurrence and composition in the essential oil of Thymus vulgaris chemotypes. Journal of Chemical Ecology 29(4): 859–880. DOI: 10.1023/A:1022927615442
Thomson S.A., Pyle R.L., Ahyong S.T., Alonso-Zarazaga M., Ammirati J., Araya J.F., Ascher J.S., Audisio T.L., Azevedo-Santos V.M., Bailly N., Baker W.J., Balke M., Barclay M.V.L., Barrett R.L., Benine R.C., Bickerstaff J.R.M., Bouchard P., Bour R., Bourgoin T., Boyko C.B., Breure A.S.H., Brothers D.J., Byng J.W., Campbell D., Ceríaco L.M.P., Cernák I., Cerretti P., Chang C.H., Cho S., Copus J.M. et al. 2018. Taxonomy based on science is necessary for global conservation. PLoS Biology 16(3): e2005075. DOI: 10.1371/journal.pbio.2005075
Tian H.Z., Han L., Zhang J., Li X., Kawahara T., Yukawa T., López-Pujol J., Kumar P., Chung M.G., Chung M.Y. 2018. Genetic diversity in the endangered terrestrial orchid Cypripedium japonicum in East Asia: Insights into population history and implications for conservation. Scientific Reports 8(1): 6467. DOI: 10.1038/s41598-018-24912-z
Vakhrameeva M.G., Tatarenko I.V., Varlygina T.I., Torosyan G.K., Zagulskii M.N. 2008. Orchids of Russia and Adjacent Countries (within the borders of the former USSR). Liechtenstein: A.R.G. Gantner Verlag Ruggell. 690 p.
Ward J.H. 1963. Hierarchical grouping to optimize an objective function. Journal of the American Statistical Association 58(301): 236–244.
Waser N.M., Price M.V. 1981. Pollinator Choice and Stabilizing Selection for Flower Color in Delphinium nelsonii. Evolution 35(2): 376–390. DOI: 10.1111/j.1558-5646.1981.tb04896.x
Zhang Y.H., Sun H. 2006. Cypripedium bouffordianum (Orchidaceae), a new species from western Sichuan, China. Annales Botanici Fennici 43(6): 481–483.
Zhang Y., Zhao S., Liu D., Zhang Q., Cheng J. 2014. Flowering phenology and reproductive characteristics of Cypripedium macranthos (Orchidaceae) in China and their implication in conservation. Pakistan Journal of Botany 46(4): 1303–1308.
Zhengyi W., Raven P.H., Deyuan H. (Eds.). 2009. Flora of China: Orchidaceae. St. Louis: Science Press, Beijing & Missouri Botanical Garden Press. 570 p.
Zhivotovsky L.A. 1982. Indicators of population variability by polymorphic features. In A.V. Yablokov (Ed.): Population Phenetics. Moscow: Nauka. P. 38–45. [In Russian]